Without glitches? No.

If you hesitate for long enough, however, you'll get plenty of 'encouraging' dialogue.

"Rest assured that an independent panel of ethicists has absolved the Enrichment Center, Aperture Science employees, and all test subjects of any moral responsibility for the Companion Cube euthanizing process."

"While it has been a faithful companion, your Companion Cube cannot accompany you through the rest of the test. If it could talk - and the Enrichment Center takes this opportunity to remind you that it cannot - it would tell you to go on without it because it would rather die in a fire than become a burden to you."

"Testing cannot continue until your Companion Cube has been incinerated."

"Although the euthanizing process is remarkably painful, eight out of ten Aperture Science engineers believe that the Companion Cube is most likely incapable of feeling much pain."

"The Companion Cube cannot continue through the testing. State and Local statutory regulations prohibit it from simply remaining here, alone and companionless. You must euthanize it."

"Destroy your Companion Cube or the testing cannot continue."